1C3T

ROTAMER STRAIN AS A DETERMINANT OF PROTEIN STRUCTURAL SPECIFICITY

1C3T の概要

• エントリーDOI: 10.2210/pdb1c3t/pdb
• NMR情報: BMRB: 4663
• 分子名称: PROTEIN (1D8 UBIQUITIN) (1 entity in total)
• 機能のキーワード: protein design, hydrophobic core, packing, rotamers, roc, ubiquitin, de novo protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8576.83

構造登録者

Lazar, G.A.,Johnson, E.C.,Desjarlais, J.R.,Handel, T.M. (登録日: 1999-07-28, 公開日: 1999-08-20, 最終更新日: 2024-04-10)

主引用文献

Lazar, G.A.,Johnson, E.C.,Desjarlais, J.R.,Handel, T.M.
Rotamer strain as a determinant of protein structural specificity.
Protein Sci., 8:2598-2610, 1999

PubMed Abstract: We present direct evidence for a change in protein structural specificity due to hydrophobic core packing. High resolution structural analysis of a designed core variant of ubiquitin reveals that the protein is in slow exchange between two conformations. Examination of side-chain rotamers indicates that this dynamic response and the lower stability of the protein are coupled to greater strain and mobility in the core. The results suggest that manipulating the level of side-chain strain may be one way of fine tuning the stability and specificity of proteins.

PubMed: 10631975

実験手法

SOLUTION NMR