1C38

SOLUTION STRUCTURE OF A QUADRUPLEX FORMING DNA AND ITS INTERMEDIATE

1C38 の概要

• エントリーDOI: 10.2210/pdb1c38/pdb
• 分子名称: DNA (5'-D(*GP*GP*TP*TP*GP*GP*TP*GP*TP*GP*GP*TP*TP*GP*G)-3'), POTASSIUM ION (2 entities in total)
• 機能のキーワード: aptamer, thrombin binding, potassium, metal ion binding sites, quadruplex, intermediate, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5525.01

構造登録者

Bolton, P.H.,Marathias, V.M.,Wang, K. (登録日: 1999-07-25, 公開日: 1999-08-25, 最終更新日: 2024-05-22)

主引用文献

Marathias, V.M.,Bolton, P.H.
Structures of the potassium-saturated, 2:1, and intermediate, 1:1, forms of a quadruplex DNA.
Nucleic Acids Res., 28:1969-1977, 2000

PubMed Abstract: Potassium can stabilize the formation of chair- or edge-type quadruplex DNA structures and appears to be the only naturally occurring cation that can do so. As quadruplex DNAs may be important in the structure of telomere, centromere, triplet repeat and other DNAs, information about the details of the potassium-quadruplex DNA interactions are of interest. The structures of the 1:1 and the fully saturated, 2:1, potassium-DNA complexes of d(GGTTGGTGTGGTTGG) have been determined using the combination of experimental NMR results and restrained molecular dynamics simulations. The refined structures have been used to model the interactions at the potassium binding sites. Comparison of the 1:1 and 2:1 potassium:DNA structures indicates how potassium binding can determine the folding pattern of the DNA. In each binding site potassium interacts with the carbonyl oxygens of both the loop thymine residues and the guanine residues of the adjacent quartet.

PubMed: 10756199
DOI: 10.1093/nar/28.9.1969

実験手法

SOLUTION NMR