1C0I

CRYSTAL STRUCTURE OF D-AMINO ACID OXIDASE IN COMPLEX WITH TWO ANTHRANYLATE MOLECULES

1C0I の概要

• エントリーDOI: 10.2210/pdb1c0i/pdb
• 関連するPDBエントリー: 1C0K 1C0L 1C0P
• 分子名称: D-AMINO ACID OXIDASE, FLAVIN-ADENINE DINUCLEOTIDE, 2-AMINOBENZOIC ACID, ... (4 entities in total)
• 機能のキーワード: flavin containing protein, alpha-beta-alpha motif, oxidoreductase
• 由来する生物種: Rhodosporidium toruloides
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40674.74

構造登録者

Pollegioni, L.,Diederichs, K.,Molla, G.,Umhau, S.,Welte, W.,Ghisla, S.,Pilone, M.S. (登録日: 1999-07-16, 公開日: 2002-02-27, 最終更新日: 2023-11-15)

主引用文献

Pollegioni, L.,Diederichs, K.,Molla, G.,Umhau, S.,Welte, W.,Ghisla, S.,Pilone, M.S.
Yeast d-amino Acid oxidase: structural basis of its catalytic properties
J.Mol.Biol., 324:535-546, 2002

PubMed Abstract: The 3D structure of the flavoprotein D-amino acid oxidase (DAAO) from the yeast Rhodotorula gracilis (RgDAAO) in complex with the competitive inhibitor anthranilate was solved (resolution 1.9A) and structural features relevant for the overall conformation and for catalytic activity are described. The FAD is bound in an elongated conformation in the core of the enzyme. Two anthranilate molecules are found within the active site cavity; one is located in a funnel forming the entrance, and the second is in contact with the flavin. The anchoring of the ligand carboxylate with Arg285 and Tyr223 is found for all complexes studied. However, while the active site group Tyr238-OH interacts with the carboxylate in the case of the substrate D-alanine, of D-CF(3)-alanine, or of L-lactate, in the anthranilate complex the phenol group rotates around the C2-C3 bond thus opening the entrance of the active site, and interacts there with the second bound anthranilate. This movement serves in channeling substrate to the bottom of the active site, the locus of chemical catalysis. The absence in RgDAAO of the "lid" covering the active site, as found in mammalian DAAO, is interpreted as being at the origin of the differences in kinetic mechanism between the two enzymes. This lid has been proposed to regulate product dissociation in the latter, while the side-chain of Tyr238 might exert a similar role in RgDAAO. The more open active site architecture of RgDAAO is the origin of its much broader substrate specificity. The RgDAAO enzyme forms a homodimer with C2 symmetry that is different from that reported for mammalian D-amino acid oxidase. This different mode of aggregation probably causes the differences in stability and tightness of FAD cofactor binding between the DAAOs from different sources.

PubMed: 12445787
DOI: 10.1016/S0022-2836(02)01062-8

実験手法

X-RAY DIFFRACTION (1.9 Å)