1C01

SOLUTION STRUCTURE OF MIAMP1, A PLANT ANTIMICROBIAL PROTEIN

1C01 の概要

• エントリーDOI: 10.2210/pdb1c01/pdb
• 分子名称: ANTIMICROBIAL PEPTIDE 1 (1 entity in total)
• 機能のキーワード: greek key, beta-barrel, antimicrobial protein
• 由来する生物種: Macadamia integrifolia
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8147.05

構造登録者

McManus, A.M.,Nielsen, K.J.,Marcus, J.P.,Harrison, S.J.,Green, J.L.,Manners, J.M.,Craik, D.J. (登録日: 1999-07-13, 公開日: 2000-07-19, 最終更新日: 2024-10-09)

主引用文献

McManus, A.M.,Nielsen, K.J.,Marcus, J.P.,Harrison, S.J.,Green, J.L.,Manners, J.M.,Craik, D.J.
MiAMP1, a novel protein from Macadamia integrifolia adopts a Greek key beta-barrel fold unique amongst plant antimicrobial proteins.
J.Mol.Biol., 293:629-638, 1999

PubMed Abstract: MiAMP1 is a recently discovered 76 amino acid residue, highly basic protein from the nut kernel of Macadamia integrifolia which possesses no sequence homology to any known protein and inhibits the growth of several microbial plant pathogens in vitro while having no effect on mammalian or plant cells. It is considered to be a potentially useful tool for the genetic engineering of disease resistance in transgenic crop plants and for the design of new fungicides. The three-dimensional structure of MiAMP1 was determined through homonuclear and heteronuclear ((15)N) 2D NMR spectroscopy and subsequent simulated annealing calculations with the ultimate aim of understanding the structure-activity relationships of the protein. MiAMP1 is made up of eight beta-strands which are arranged in two Greek key motifs. These Greek key motifs associate to form a Greek key beta-barrel. This structure is unique amongst plant antimicrobial proteins and forms a new class which we term the beta-barrelins. Interestingly, the structure of MiAMP1 bears remarkable similarity to a yeast killer toxin from Williopsis mrakii. This toxin acts by inhibiting beta-glucan synthesis and thereby cell wall construction in sensitive strains of yeast. The structural similarity of MiAMP1 and WmKT, which originate from plant and fungal phyla respectively, may reflect a similar mode of action.

PubMed: 10543955
DOI: 10.1006/jmbi.1999.3163

実験手法

SOLUTION NMR