1BZG

THE SOLUTION STRUCTURE OF HUMAN PARATHYROID HORMONE-RELATED PROTEIN (1-34) IN NEAR-PHYSIOLOGICAL SOLUTION, NMR, 30 STRUCTURES

1BZG の概要

• エントリーDOI: 10.2210/pdb1bzg/pdb
• 分子名称: PARATHYROID HORMONE-RELATED PROTEIN (1 entity in total)
• 機能のキーワード: human peptide hormone, stimulating intracellular camp formation, serum calcium level, humoral hypercalcemia of malignancy, solution structure, pthrp, pth, hormone
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4027.64

構造登録者

Weidler, M.,Marx, U.C.,Seidel, G.,Roesch, P. (登録日: 1998-10-28, 公開日: 1999-05-18, 最終更新日: 2024-05-22)

主引用文献

Weidler, M.,Marx, U.C.,Seidel, G.,Schafer, W.,Hoffmann, E.,Esswein, A.,Rosch, P.
The structure of human parathyroid hormone-related protein(1-34) in near-physiological solution.
FEBS Lett., 444:239-244, 1999

PubMed Abstract: Parathyroid hormone-related protein plays a major role in the pathogenesis of humoral hypercalcemia of malignancy. Under normal physiological conditions, parathyroid hormone-related protein is produced in a wide variety of tissues and acts in an autocrine or paracrine fashion. Parathyroid hormone-related protein and parathyroid hormone bind to and activate the same G-protein-coupled receptor. Here we present the structure of the biologically active NH2-terminal domain of human parathyroid hormone-related protein(1-34) in near-physiological solution in the absence of crowding reagents as determined by two-dimensional proton magnetic resonance spectroscopy. An improved strategy for structure calculation revealed the presence of two helices, His-5-Leu-8 and Gln-16-Leu-27, connected by a flexible linker. The parathyroid hormone-related protein(1-34) structure and the structure of human parathyroid hormone(1-37) as well as human parathyroid hormone(1-34) are highly similar, except for the well defined turn, His-14-Ser-17, present in parathyroid hormone. Thus, the similarity of the binding affinities of parathyroid hormone and parathyroid hormone-related protein to their common receptor may be based on their structural similarity.

PubMed: 10050767
DOI: 10.1016/S0014-5793(98)01658-5

実験手法

SOLUTION NMR