1BYV

GLYCOSYLATED EEL CALCITONIN

1BYV の概要

• エントリーDOI: 10.2210/pdb1byv/pdb
• NMR情報: BMRB: 4260
• 分子名称: PROTEIN (CALCITONIN), 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• 機能のキーワード: horomone, calcium-regulator, osteoporosis, hormone-growth factor complex, hormone/growth factor
• 由来する生物種: Anguilla japonica (Japanese eel)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3641.11

構造登録者

Hashimoto, Y.,Toma, K.,Nishikido, J.,Yamamoto, K.,Haneda, K.,Inazu, T.,Valentine, K.G.,Opella, S.J. (登録日: 1998-10-16, 公開日: 1998-10-28, 最終更新日: 2020-07-29)

主引用文献

Hashimoto, Y.,Toma, K.,Nishikido, J.,Yamamoto, K.,Haneda, K.,Inazu, T.,Valentine, K.G.,Opella, S.J.
Effects of glycosylation on the structure and dynamics of eel calcitonin in micelles and lipid bilayers determined by nuclear magnetic resonance spectroscopy.
Biochemistry, 38:8377-8384, 1999

PubMed Abstract: The three-dimensional structures of eel calcitonin (CT) and two glycosylated CT derivatives, [Asn(GlcNAc)3]-CT (CT-GlcNAc) and [Asn(Man6-GlcNAc2)3]-CT (CT-M6), in micelles were determined by solution NMR spectroscopy. The topologies of these peptides associated with oriented lipid bilayers were determined with solid-state NMR. All of the peptides were found to have an identical conformation in micelles characterized by an amphipathic alpha-helix consisting of residues Ser5 through Leu19 followed by an unstructured region at the C-terminus. The overall conformation of the peptide moiety was not affected by the glycosylation. Nevertheless, comparison of the relative exchange rates of the Leu12 amide proton might suggest the possibility that fluctuations of the alpha-helix are reduced by glycosylation. The presence of NOEs between the carbohydrate and the peptide moieties of CT-GlcNAc and CT-M6 and the amide proton chemical shift data suggested that the carbohydrate interacted with the peptide, and this might account for the conformational stabilization of the alpha-helix. Both the unmodified CT and the glycosylated CT were found to have orientations with their helix axes parallel to the plane of the lipid bilayers by solid-state NMR spectroscopy.

PubMed: 10387083
DOI: 10.1021/bi983018j

実験手法

SOLUTION NMR