1BYJ

GENTAMICIN C1A A-SITE COMPLEX

1BYJ の概要

• エントリーDOI: 10.2210/pdb1byj/pdb
• 分子名称: RNA (16S RNA), 2,6-diamino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose, 3,5-DIAMINO-CYCLOHEXANOL, ... (4 entities in total)
• 機能のキーワード: complex (aminoglycoside-ribosomal rna), rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9109.76

構造登録者

Yoshizawa, S.,Fourmy, D.,Puglisi, J.D. (登録日: 1998-10-16, 公開日: 1999-10-29, 最終更新日: 2023-12-27)

主引用文献

Yoshizawa, S.,Fourmy, D.,Puglisi, J.D.
Structural origins of gentamicin antibiotic action.
EMBO J., 17:6437-6448, 1998

PubMed Abstract: Aminoglycoside antibiotics that bind to the ribosomal A site cause misreading of the genetic code and inhibit translocation. The clinically important aminoglycoside, gentamicin C, is a mixture of three components. Binding of each gentamicin component to the ribosome and to a model RNA oligonucleotide was studied biochemically and the structure of the RNA complexed to gentamicin C1a was solved using magnetic resonance nuclear spectroscopy. Gentamicin C1a binds in the major groove of the RNA. Rings I and II of gentamicin direct specific RNA-drug interactions. Ring III of gentamicin, which distinguishes this subclass of aminoglycosides, also directs specific RNA interactions with conserved base pairs. The structure leads to a general model for specific ribosome recognition by aminoglycoside antibiotics and a possible mechanism for translational inhibition and miscoding. This study provides a structural rationale for chemical synthesis of novel aminoglycosides.

PubMed: 9822590
DOI: 10.1093/emboj/17.22.6437

実験手法

SOLUTION NMR