1BY1

DBL homology domain from beta-PIX

1BY1 の概要

• エントリーDOI: 10.2210/pdb1by1/pdb
• 分子名称: PROTEIN (PIX) (1 entity in total)
• 機能のキーワード: rho-gtpase exchange factor, transport protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23972.47

構造登録者

Aghazadeh, B.,Zhu, K.,Kubiseski, T.J.,Liu, G.A.,Pawson, T.,Zheng, Y.,Rosen, M.K. (登録日: 1998-10-22, 公開日: 1999-10-24, 最終更新日: 2023-12-27)

主引用文献

Aghazadeh, B.,Zhu, K.,Kubiseski, T.J.,Liu, G.A.,Pawson, T.,Zheng, Y.,Rosen, M.K.
Structure and Mutagenesis of the Dbl Homology Domain
Nat.Struct.Biol., 5:1098-1107, 1998

PubMed Abstract: Guanine nucleotide exchange factors in the Dbl family activate Rho GTPases by accelerating dissociation of bound GDP, promoting acquisition of the GTP-bound state. Dbl proteins possess a approximately 200 residue catalytic Dbl-homology (DH) domain, that is arranged in tandem with a C-terminal pleckstrin homology (PH) domain in nearly all cases. Here we report the solution structure of the DH domain of human PAK-interacting exchange protein (betaPIX). The domain is composed of 11 alpha-helices that form a flattened, elongated bundle. The structure explains a large body of mutagenesis data, which, along with sequence comparisons, identify the GTPase interaction site as a surface formed by three conserved helices near the center of one face of the domain. Proximity of the site to the DH C-terminus suggests a means by which PH-ligand interactions may be coupled to DH-GTPase interactions to regulate signaling through the Dbl proteins in vivo.

PubMed: 9846881
DOI: 10.1038/4209

実験手法

SOLUTION NMR