1BVX

THE 1.8 A STRUCTURE OF GEL GROWN TETRAGONAL HEN EGG WHITE LYSOZYME

Summary for 1BVX

• Entry DOI: 10.2210/pdb1bvx/pdb
• Descriptor: PROTEIN (LYSOZYME) (2 entities in total)
• Functional Keywords: lysozyme, hydrolase
• Biological source: Gallus gallus (chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 14331.16

Authors

Dong, J.,Boggon, T.J.,Chayen, N.E.,Raftery, J.,Bi, R.C.,Helliwell, J.R. (deposition date: 1998-09-18, release date: 1998-09-23, Last modification date: 2024-10-30)

Primary citation

Dong, J.,Boggon, T.J.,Chayen, N.E.,Raftery, J.,Bi, R.C.,Helliwell, J.R.
Bound-solvent structures for microgravity-, ground control-, gel- and microbatch-grown hen egg-white lysozyme crystals at 1.8 A resolution.
Acta Crystallogr.,Sect.D, 55:745-752, 1999

PubMed Abstract: A number of methods can be used to improve the stability of the protein crystal-growth environment, including growth in microgravity without an air-liquid phase boundary, growth in gels and growth under oil ('microbatch'). In this study, X-ray data has been collected from and structures refined for crystals of hen egg-white lysozyme (HEWL) grown using four different methods, liquid-liquid dialysis on Earth and in microgravity using the European Space Agency's (ESA) Advanced Protein Crystallization Facility (APCF) on board the NASA Space Shuttle Life and Microgravity Spacelab (LMS) mission (STS-78), crystallization in agarose gel using a tube liquid-gel diffusion method and crystallization in microbatch under oil. A comparison of the overall quality of the X-ray data, the protein structures and especially the bound-water structures has been carried out at 1.8 A. The lysozyme protein structures corresponding to these four different crystallization methods remain similar. A small improvement in the bound-solvent structure is seen in lysozyme crystals grown in microgravity by liquid-liquid dialysis, which has a more stable fluid physics state in microgravity, and is consistent with a better formed protein crystal in microgravity.

PubMed: 10089304
DOI: 10.1107/S0907444998016047

Experimental method

X-RAY DIFFRACTION (1.8 Å)