1BUO

BTB DOMAIN FROM PLZF

1BUO の概要

• エントリーDOI: 10.2210/pdb1buo/pdb
• 分子名称: PROTEIN (PROMYELOCYTIC LEUKEMIA ZINC FINGER PROTEIN PLZF) (2 entities in total)
• 機能のキーワード: protein-protein interaction domain, transcriptional repressor, zinc-finger protein, protein structure, promyelocytic leukemia, gene regulation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q05516
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13927.04

構造登録者

Ahmad, K.F.,Engel, C.K.,Prive, G.G. (登録日: 1998-09-04, 公開日: 1998-10-14, 最終更新日: 2024-02-07)

主引用文献

Ahmad, K.F.,Engel, C.K.,Prive, G.G.
Crystal structure of the BTB domain from PLZF.
Proc.Natl.Acad.Sci.USA, 95:12123-12128, 1998

PubMed Abstract: The BTB domain (also known as the POZ domain) is an evolutionarily conserved protein-protein interaction motif found at the N terminus of 5-10% of C2H2-type zinc-finger transcription factors, as well as in some actin-associated proteins bearing the kelch motif. Many BTB proteins are transcriptional regulators that mediate gene expression through the control of chromatin conformation. In the human promyelocytic leukemia zinc finger (PLZF) protein, the BTB domain has transcriptional repression activity, directs the protein to a nuclear punctate pattern, and interacts with components of the histone deacetylase complex. The association of the PLZF BTB domain with the histone deacetylase complex provides a mechanism of linking the transcription factor with enzymatic activities that regulate chromatin conformation. The crystal structure of the BTB domain of PLZF was determined at 1.9 A resolution and reveals a tightly intertwined dimer with an extensive hydrophobic interface. Approximately one-quarter of the monomer surface area is involved in the dimer intermolecular contact. These features are typical of obligate homodimers, and we expect the full-length PLZF protein to exist as a branched transcription factor with two C-terminal DNA-binding regions. A surface-exposed groove lined with conserved amino acids is formed at the dimer interface, suggestive of a peptide-binding site. This groove may represent the site of interaction of the PLZF BTB domain with nuclear corepressors or other nuclear proteins.

PubMed: 9770450
DOI: 10.1073/pnas.95.21.12123

実験手法

X-RAY DIFFRACTION (1.9 Å)