1BSW

ACUTOLYSIN A FROM SNAKE VENOM OF AGKISTRODON ACUTUS AT PH 7.5

1BSW の概要

• エントリーDOI: 10.2210/pdb1bsw/pdb
• 関連するPDBエントリー: 1BUD
• 分子名称: PROTEIN (ACUTOLYSIN A), ZINC ION, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: metalloproteinase, snake venom, mmp, metal binding protein
• 由来する生物種: Deinagkistrodon acutus (Chinese moccasin)
• 細胞内の位置: Secreted: Q9PW35
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21956.22

構造登録者

Gong, W.,Zhu, X.,Liu, S.,Teng, M.,Niu, L. (登録日: 1998-08-31, 公開日: 1999-08-26, 最終更新日: 2024-11-13)

主引用文献

Gong, W.,Zhu, X.,Liu, S.,Teng, M.,Niu, L.
Crystal structures of acutolysin A, a three-disulfide hemorrhagic zinc metalloproteinase from the snake venom of Agkistrodon acutus.
J.Mol.Biol., 283:657-668, 1998

PubMed Abstract: Acutolysin A alias AaHI, a 22 kDa hemorrhagic toxin isolated from the snake venom of Agkistrodon acutus, is a member of the adamalysin subfamily of the metzincin family and is a snake venom zinc metalloproteinase possessing only one catalytic domain. Acutolysin A was found to have a high-activity and a low-activity under weakly alkaline and acidic conditions, respectively. With the adamalysin II structure as the initial trial-and-error model, the crystal structures were solved to the final crystallographic R-factors of 0. 168 and 0.171, against the diffraction data of crystals grown under pH 5.0 and pH 7.5 conditions to 1.9 A and 1.95 A resolution, respectively. One zinc ion, binding in the active-site, one structural calcium ion and some water molecules were localized in both of the structures. The catalytic zinc ion is coordinated in a tetrahedral manner with one catalytic water molecule anchoring to an intermediate glutamic acid residue (Glu143) and three imidazole Nepsilon2 atoms of His142, His146 and His152 in the highly conserved sequence H142E143XXH146XXGXXH152. There are two new disulfide bridges (Cys157-Cys181 and Cys159-Cys164) in acutolysin A in addition to the highly conserved disulfide bridge Cys117-Cys197. The calcium ion occurs on the molecular surface. The superposition showed that there was no significant conformational changes between the two structures except for a few slight changes of some flexible residue side-chains on the molecular surface, terminal residues and the active-site cleft. The average contact distance between the catalytic water molecule and oxygen atoms of the Glu143 carboxylate group in the weakly alkaline structure was also found to be closer than that in the weakly acidic structure. By comparing the available structural information of the members of the adamalysin subfamily, it seems that, when lowering the pH value, the polarization capability of the Glu143 carboxylate group to the catalytic water molecule become weaker, which might be the structural reason why the snake venom metalloproteinases are inactive or have a low activity under acidic conditions.

PubMed: 9784374
DOI: 10.1006/jmbi.1998.2110

実験手法

X-RAY DIFFRACTION (1.95 Å)