1BRP

CRYSTAL STRUCTURE OF THE TRIGONAL FORM OF HUMAN PLASMA RETINOL-BINDING PROTEIN AT 2.5 ANGSTROMS RESOLUTION

1BRP の概要

• エントリーDOI: 10.2210/pdb1brp/pdb
• 分子名称: RETINOL BINDING PROTEIN, RETINOL (3 entities in total)
• 機能のキーワード: retinol transport
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted : P02753
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21270.90

構造登録者

Zanotti, G.,Monaco, H.L. (登録日: 1992-07-27, 公開日: 1994-01-31, 最終更新日: 2024-10-30)

主引用文献

Zanotti, G.,Ottonello, S.,Berni, R.,Monaco, H.L.
Crystal structure of the trigonal form of human plasma retinol-binding protein at 2.5 A resolution.
J.Mol.Biol., 230:613-624, 1993

PubMed Abstract: The three-dimensional structures of the liganded and unliganded forms of human plasma retinol binding protein (RBP) in the trigonal crystal form have been solved at 2.5 A resolution. The final model of RBP complexed with retinol (holoRBP, space group R3, a = b = 104.0 A, c = 74.4 A) has a crystallographic R factor of 0.176 for 9652 reflections. The unliganded form, obtained through a purification procedure which included steps based on hydrophobic interaction chromatography, crystallized isomorphously with holoRBP and its structure has been refined to an R factor of 0.190 for 9614 reflections. The structure of the trigonal holo protein is quite similar to that of the orthorhombic form: the root-mean-square deviation of all the equivalent alpha-carbons in the two chains is 0.53 A. The structural comparison between the liganded and unliganded forms of RBP in the crystal did not reveal gross conformational changes. The most significant difference between the two forms of the protein is a conformational change involving residues from 34 to 37. In this region, the movements of side-chains of Leu35 and Phe36 are most noticeable. In particular, in the unliganded form the side-chain ring of the latter residue is in the place previously occupied by the alcoholic moiety of retinol. Our data are consistent with a model in which a region comprising these residues and at least part of the opening of the beta-barrel is involved in the recognition between RBP and transthyretin. In the case of the unliganded form, the central cavity, that is occupied by the vitamin in the two human crystalline holoRBPs, is filled by electron density that, at the present resolution, we interpret as solvent.

PubMed: 8464067
DOI: 10.1006/jmbi.1993.1173

実験手法

X-RAY DIFFRACTION (2.5 Å)