1BQF

GROWTH-BLOCKING PEPTIDE (GBP) FROM PSEUDALETIA SEPARATA

1BQF の概要

• エントリーDOI: 10.2210/pdb1bqf/pdb
• 分子名称: PROTEIN (GROWTH-BLOCKING PEPTIDE) (1 entity in total)
• 機能のキーワード: growth factor, hormone-growth factor complex, hormone/growth factor
• 由来する生物種: Apanteles kariyai
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2788.12

構造登録者

Aizawa, T.,Fujitani, N.,Hayakawa, Y.,Ohnishi, A.,Ohkubo, T.,Kwano, K.,Hikichi, K.,Nitta, K. (登録日: 1998-08-09, 公開日: 1998-12-09, 最終更新日: 2024-11-13)

主引用文献

Aizawa, T.,Fujitani, N.,Hayakawa, Y.,Ohnishi, A.,Ohkubo, T.,Kumaki, Y.,Kawano, K.,Hikichi, K.,Nitta, K.
Solution structure of an insect growth factor, growth-blocking peptide.
J.Biol.Chem., 274:1887-1890, 1999

PubMed Abstract: Growth-blocking peptide (GBP) is an insect growth factor consisting of 25 amino acid residues that retards the development of lepidopteran larvae at high concentration while it stimulates larval growth at low concentration. In this study, we determined the solution structure of GBP by two-dimensional 1H NMR spectroscopy. The structure contains a short segment of double-stranded beta-sheet involving residues 11-13 and 19-21 and a type-II beta-turn in the loop region (residues 8-11), whereas the N and C termini are disordered. This is the first report of the three-dimensional structure of the peptiderigic insect growth factor, and the structure of the well defined region of GBP was found to share similarity with that of the C-terminal domain of the epidermal growth factor (EGF). Because GBP has been reported to stimulate DNA synthesis of not only insect cells but also human keratinocyte cells at the same level with EGF, the structural similarity between GBP and EGF may lead to the interaction of GBP to EGF receptor.

PubMed: 9890941
DOI: 10.1074/jbc.274.4.1887

実験手法

SOLUTION NMR