1BQ5

NITRITE REDUCTASE FROM ALCALIGENES XYLOSOXIDANS GIFU 1051

Summary for 1BQ5

• Entry DOI: 10.2210/pdb1bq5/pdb
• Descriptor: NITRITE REDUCTASE, COPPER (II) ION (3 entities in total)
• Functional Keywords: oxidoreductase, nitrite reductase, cuproprotein
• Biological source: Achromobacter xylosoxidans
• Total number of polymer chains: 1
• Total formula weight: 37256.20

Authors

Inoue, T.,Gotowda, M.,Deligeer,Suzuki, S.,Kataoka, K.,Yamaguchi, K.,Watanabe, H.,Goho, M.,Yasushi, K.A.I. (deposition date: 1998-08-21, release date: 1999-08-21, Last modification date: 2024-02-07)

Primary citation

Inoue, T.,Gotowda, M.,Deligeer,Kataoka, K.,Yamaguchi, K.,Suzuki, S.,Watanabe, H.,Gohow, M.,Kai, Y.
Type 1 Cu structure of blue nitrite reductase from Alcaligenes xylosoxidans GIFU 1051 at 2.05 A resolution: comparison of blue and green nitrite reductases.
J.Biochem.(Tokyo), 124:876-879, 1998

PubMed Abstract: The crystal structure of the blue nitrite reductase from Alcaligenes xylosoxidans GIFU 1051 (AxgNIR) has been determined at 2.05 A resolution. AxgNIR contains both type 1 and 2 Cu sites, the geometry of the former being distorted tetrahedral. The superpositioning of the type 1 Cu sites in the blue enzyme and a green nitrite reductase revealed that the orientation of the Met150 side chain differed. The deviation of the Sdelta(Met150) atom from the axial position of the NNS plane formed by two Ndelta(His95 and His145) and one Sgamma(Cys136) atom caused the difference in the colors of the enzymes, i.e. blue and green.

PubMed: 9792907

Experimental method

X-RAY DIFFRACTION (2.05 Å)