1BO1

PHOSPHATIDYLINOSITOL PHOSPHATE KINASE TYPE II BETA

1BO1 の概要

• エントリーDOI: 10.2210/pdb1bo1/pdb
• 分子名称: PROTEIN (PHOSPHATIDYLINOSITOL PHOSPHATE KINASE IIBETA) (2 entities in total)
• 機能のキーワード: lipid signaling, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 94895.85

構造登録者

Rao, V.D.,Misra, S.,Boronenkov, I.V.,Anderson, R.A.,Hurley, J.H. (登録日: 1998-08-02, 公開日: 1998-10-07, 最終更新日: 2024-02-07)

主引用文献

Rao, V.D.,Misra, S.,Boronenkov, I.V.,Anderson, R.A.,Hurley, J.H.
Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation.
Cell(Cambridge,Mass.), 94:829-839, 1998

PubMed Abstract: Phosphoinositide kinases play central roles in signal transduction by phosphorylating the inositol ring at specific positions. The structure of one such enzyme, type IIbeta phosphatidylinositol phosphate kinase, reveals a protein kinase ATP-binding core and demonstrates that all phosphoinositide kinases belong to one superfamily. The enzyme is a disc-shaped homodimer with a 33 x 48 A basic flat face that suggests an electrostatic mechanism for plasma membrane targeting. Conserved basic residues form a putative phosphatidylinositol phosphate specificity site. The substrate-binding site is open on one side, consistent with dual specificity for phosphatidylinositol 3- and 5-phosphates. A modeled complex with membrane-bound substrate and ATP shows how a phosphoinositide kinase can phosphorylate its substrate in situ at the membrane interface.

PubMed: 9753329
DOI: 10.1016/S0092-8674(00)81741-9

実験手法

X-RAY DIFFRACTION (3 Å)