1BN8

BACILLUS SUBTILIS PECTATE LYASE

1BN8 の概要

• エントリーDOI: 10.2210/pdb1bn8/pdb
• 分子名称: PROTEIN (PECTATE LYASE), CALCIUM ION (3 entities in total)
• 機能のキーワード: parallel beta-helix, lyase
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Secreted: P39116
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45587.87

構造登録者

Pickersgill, R.,Harris, G.,Jenkins, J. (登録日: 1998-07-31, 公開日: 1998-08-05, 最終更新日: 2024-02-07)

主引用文献

Pickersgill, R.,Jenkins, J.,Harris, G.,Nasser, W.,Robert-Baudouy, J.
The structure of Bacillus subtilis pectate lyase in complex with calcium.
Nat.Struct.Biol., 1:717-723, 1994

PubMed Abstract: We have solved the structure of the Bacillus subtilis pectate lyase (BsPel) in complex with calcium. The structure consists of a parallel beta-helix domain and a loop region. The alpha L-bounded beta-strand seen in BsPel is a new element of protein structure and its frequent occurrence suggests it is an important characteristic of the parallel beta-helix. A pronounced cleft is formed between the loops and the parallel beta-helix domain and we propose that this is the active site cleft. Calcium, essential for the activity of the enzyme, binds at the bottom of this cleft and an arginine residue close to the calcium, which is conserved across all pectin and pectate lyases, may be involved in catalysis.

PubMed: 7634076
DOI: 10.1038/nsb1094-717

実験手法

X-RAY DIFFRACTION (1.8 Å)