1BLV

SOLUTION STRUCTURE OF OXIDIZED RAT MICROSOMAL CYTOCHROME B5 IN THE PRESENCE OF 2 M GUANIDINIUM CHLORIDE: MONITORING THE EARLY STEPS IN PROTEIN UNFOLDING

1BLV の概要

• エントリーDOI: 10.2210/pdb1blv/pdb
• 分子名称: PROTEIN (CYTOCHROME B5), PROTOPORPHYRIN IX CONTAINING FE (2 entities in total)
• 機能のキーワード: protein unfolding, nmr solution structure, cytochrome b5, electron transport
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side: P00173
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11430.39

構造登録者

Arnesano, F.,Banci, L.,Bertini, I.,Koulougliotis, D. (登録日: 1998-07-21, 公開日: 1998-07-29, 最終更新日: 2024-05-22)

主引用文献

Arnesano, F.,Banci, L.,Bertini, I.,Koulougliotis, D.
Solution structure of oxidized rat microsomal cytochrome b5 in the presence of 2 M guanidinium chloride: monitoring the early steps in protein unfolding.
Biochemistry, 37:17082-17092, 1998

PubMed Abstract: One- and two-dimensional proton NMR spectroscopy has been employed in order to study the denaturation effect of guanidinium chloride (GdmCl) on the oxidized state of the A-form of rat microsomal cytochrome b5 (cyt b5). The protein rapidly starts losing the heme at denaturant concentrations larger than approximately 2.0 M and a largely unfolded protein is eventually obtained. An estimate of the unfolding kinetics is obtained and, by use of a two-state model (folded left and right arrow unfolded), a value for DeltaG degrees. Below this concentration, small (PubMed: 9836603
DOI: 10.1021/bi981546p

実験手法

SOLUTION NMR