1BLQ

STRUCTURE AND INTERACTION SITE OF THE REGULATORY DOMAIN OF TROPONIN-C WHEN COMPLEXED WITH THE 96-148 REGION OF TROPONIN-I, NMR, 29 STRUCTURES

1BLQ の概要

• エントリーDOI: 10.2210/pdb1blq/pdb
• NMR情報: BMRB: 4401
• 分子名称: N-TROPONIN C (1 entity in total)
• 機能のキーワード: calcium-binding, regulation, troponin c, skeletal muscle, contraction, calcium-binding protein
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9984.08

構造登録者

Mckay, R.T.,Pearlstone, J.R.,Corson, D.C.,Gagne, S.M.,Smillie, L.B.,Sykes, B.D. (登録日: 1998-07-19, 公開日: 1999-01-13, 最終更新日: 2024-05-22)

主引用文献

McKay, R.T.,Pearlstone, J.R.,Corson, D.C.,Gagne, S.M.,Smillie, L.B.,Sykes, B.D.
Structure and interaction site of the regulatory domain of troponin-C when complexed with the 96-148 region of troponin-I.
Biochemistry, 37:12419-12430, 1998

PubMed Abstract: The structure of the regulatory domain of chicken skeletal troponin-C (residues 1-90) when complexed with the major inhibitory region (residues 96-148) of chicken skeletal troponin-I was determined using multinuclear, multidimensional NMR spectroscopy. This complex represents the first interaction formed between the regulatory domain of troponin-C and troponin-I after calcium binding in the regulation of muscle contraction. The stoichiometry of the complex was determined to be 1:1, with a dissociation constant in the 1-40 microM range. The structure of troponin-C in the complex was calculated from 1039 NMR distance and 111 dihedral angle restraints. When compared to the structure of this domain in the calcium saturated "open" form but in the absence of troponin-I, the bound structure appears to be slightly more "closed". The troponin-I peptide-binding site was found to be in the hydrophobic pocket of calcium saturated troponin-C, using edited/filtered NMR experiments and chemical shift mapping of changes induced in the regulatory domain upon peptide binding. The troponin-I peptide (residues 96-148) was found to bind to the regulatory domain of troponin-C very similarly, but not identically, to a shorter troponin-I peptide (region 115-131) thought to represent the major interaction site of troponin-I for this domain of troponin-C.

PubMed: 9730814
DOI: 10.1021/bi9809019

実験手法

SOLUTION NMR