1BJF

CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS

Summary for 1BJF

Entry DOI	10.2210/pdb1bjf/pdb
Descriptor	NEUROCALCIN DELTA, CALCIUM ION (3 entities in total)
Functional Keywords	calcium-binding, myristoylation, neuronal specific guanylate cyclase activator
Biological source	Bos taurus (cattle)
Total number of polymer chains	2
Total formula weight	44788.98
Authors	Vijay-Kumar, S.,Kumar, V.D. (deposition date: 1998-06-25, release date: 1999-07-22, Last modification date: 2024-02-07)
Primary citation	Vijay-Kumar, S.,Kumar, V.D. Crystal structure of recombinant bovine neurocalcin. Nat.Struct.Biol., 6:80-88, 1999 Cited by PubMed Abstract: The crystal structure of calcium-bound unmyristoylated bovine neurocalcin from Escherichia coli has been determined at 2.4 A resolution. The three-dimensional structure reveals a highly compact structure consisting of: (i) two pairs of calcium-binding EF-hands (EF1-EF2 and EF3-EF4); (ii) a calcium ion bound at EF2, EF3 and EF4 sites; and (iii) an EF1-hand that is disabled from calcium-binding due to a Cys-Pro sequence in the Ca2+-binding loop. The crystal structure of neurocalcin resembles photoreceptor recoverin in overall topology, however its EF2- and EF4-hands differ. Recently, neurocalcin in the calcium-bound state has been shown to stimulate mammalian rod outer segment membrane guanylate cyclase. A possible site for cyclase activity based on the three-dimensional structure is discussed. PubMed: 9886296 DOI: 10.1038/4956 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.4 Å)

Structure validation