1BJF

CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS

1BJF の概要

• エントリーDOI: 10.2210/pdb1bjf/pdb
• 分子名称: NEUROCALCIN DELTA, CALCIUM ION (3 entities in total)
• 機能のキーワード: calcium-binding, myristoylation, neuronal specific guanylate cyclase activator
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44788.98

構造登録者

Vijay-Kumar, S.,Kumar, V.D. (登録日: 1998-06-25, 公開日: 1999-07-22, 最終更新日: 2024-02-07)

主引用文献

Vijay-Kumar, S.,Kumar, V.D.
Crystal structure of recombinant bovine neurocalcin.
Nat.Struct.Biol., 6:80-88, 1999

PubMed Abstract: The crystal structure of calcium-bound unmyristoylated bovine neurocalcin from Escherichia coli has been determined at 2.4 A resolution. The three-dimensional structure reveals a highly compact structure consisting of: (i) two pairs of calcium-binding EF-hands (EF1-EF2 and EF3-EF4); (ii) a calcium ion bound at EF2, EF3 and EF4 sites; and (iii) an EF1-hand that is disabled from calcium-binding due to a Cys-Pro sequence in the Ca2+-binding loop. The crystal structure of neurocalcin resembles photoreceptor recoverin in overall topology, however its EF2- and EF4-hands differ. Recently, neurocalcin in the calcium-bound state has been shown to stimulate mammalian rod outer segment membrane guanylate cyclase. A possible site for cyclase activity based on the three-dimensional structure is discussed.

PubMed: 9886296
DOI: 10.1038/4956

実験手法

X-RAY DIFFRACTION (2.4 Å)