1BIT
THE CRYSTAL STRUCTURE OF ANIONIC SALMON TRYPSIN IN A SECOND CRYSTAL FORM
Summary for 1BIT
| Entry DOI | 10.2210/pdb1bit/pdb |
| Descriptor | TRYPSIN, CALCIUM ION, SULFATE ION, ... (5 entities in total) |
| Functional Keywords | serine proteinase |
| Biological source | Salmo salar (Atlantic salmon) |
| Total number of polymer chains | 1 |
| Total formula weight | 25778.94 |
| Authors | Berglund, G.I. (deposition date: 1994-08-26, release date: 1994-11-01, Last modification date: 2019-08-14) |
| Primary citation | Berglund, G.I.,Smalas, A.O.,Hordvik, A.,Willassen, N.P. Structure of anionic salmon trypsin in a second crystal form. Acta Crystallogr.,Sect.D, 51:725-730, 1995 Cited by PubMed Abstract: Anionic salmon trypsin in a second crystal form (ST-IIB) has been refined at 1.83 A, resolution. The crystals are orthorhombic and belong to space group P2(1)2(1)2 with lattice parameters a = 77.09, b = 82.33 and c = 31.16 A. The present structure has been compared to salmon trypsin as it appears in a previously reported crystal form (ST-IIA) with cell dimensions a = 61.95, b = 84.33 and c = 39.11 A [Smalås & Hordvik (1993). Acta Cryst. D49, 318-330]. The presence of a sulfate group involved in several hydrogen bonds to active-site residues, and the location of an additional benzamidine site in the crystal lattice, are the most striking differences between the present and the previous structure. Superposition of main-chain atoms in the two structures give an overall r.m.s. difference of 0.26 A, with the main differences located to areas with different molecular packing. The overall coordinate error is estimated to be between 0.20 and 0.25 A, by the method of Luzzati. PubMed: 15299802DOI: 10.1107/S0907444995000333 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.83 Å) |
Structure validation
Download full validation report
