1BIT

THE CRYSTAL STRUCTURE OF ANIONIC SALMON TRYPSIN IN A SECOND CRYSTAL FORM

1BIT の概要

• エントリーDOI: 10.2210/pdb1bit/pdb
• 分子名称: TRYPSIN, CALCIUM ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: serine proteinase
• 由来する生物種: Salmo salar (Atlantic salmon)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25778.94

構造登録者

Berglund, G.I. (登録日: 1994-08-26, 公開日: 1994-11-01, 最終更新日: 2024-11-20)

主引用文献

Berglund, G.I.,Smalas, A.O.,Hordvik, A.,Willassen, N.P.
Structure of anionic salmon trypsin in a second crystal form.
Acta Crystallogr.,Sect.D, 51:725-730, 1995

PubMed Abstract: Anionic salmon trypsin in a second crystal form (ST-IIB) has been refined at 1.83 A, resolution. The crystals are orthorhombic and belong to space group P2(1)2(1)2 with lattice parameters a = 77.09, b = 82.33 and c = 31.16 A. The present structure has been compared to salmon trypsin as it appears in a previously reported crystal form (ST-IIA) with cell dimensions a = 61.95, b = 84.33 and c = 39.11 A [Smalås & Hordvik (1993). Acta Cryst. D49, 318-330]. The presence of a sulfate group involved in several hydrogen bonds to active-site residues, and the location of an additional benzamidine site in the crystal lattice, are the most striking differences between the present and the previous structure. Superposition of main-chain atoms in the two structures give an overall r.m.s. difference of 0.26 A, with the main differences located to areas with different molecular packing. The overall coordinate error is estimated to be between 0.20 and 0.25 A, by the method of Luzzati.

PubMed: 15299802
DOI: 10.1107/S0907444995000333

実験手法

X-RAY DIFFRACTION (1.83 Å)