1BE1

GLUTAMATE MUTASE (B12-BINDING SUBUNIT), NMR, MINIMIZED AVERAGE STRUCTURE

1BE1 の概要

• エントリーDOI: 10.2210/pdb1be1/pdb
• 分子名称: GLUTAMATE MUTASE (1 entity in total)
• 機能のキーワード: isomerase, glutamate mutase, b12-binding subunit
• 由来する生物種: Clostridium tetanomorphum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14763.86

構造登録者

Tollinger, M.,Konrat, R.,Hilbert, B.H.,Marsh, E.N.G.,Kraeutler, B. (登録日: 1998-05-19, 公開日: 1998-08-26, 最終更新日: 2024-05-22)

主引用文献

Tollinger, M.,Konrat, R.,Hilbert, B.H.,Marsh, E.N.,Krautler, B.
How a protein prepares for B12 binding: structure and dynamics of the B12-binding subunit of glutamate mutase from Clostridium tetanomorphum
Structure, 6:1021-1033, 1998

PubMed Abstract: Glutamate mutase is an adenosylcobamide (coenzyme B12) dependent enzyme that catalyzes the reversible rearrangement of (2S)-glutamate to (2S,3S)-3-methylaspartate. The enzyme from Clostridium tetanomorphum comprises two subunits (of 53.7 and 14.8 kDa) and in its active form appears to be an alpha 2 beta 2 tetramer. The smaller subunit, termed MutS, has been characterized as the B12-binding component. Knowledge on the structure of a B12-binding apoenzyme does not exist.

PubMed: 9739092
DOI: 10.1016/S0969-2126(98)00103-8

実験手法

SOLUTION NMR