1BDJ

COMPLEX STRUCTURE OF HPT DOMAIN AND CHEY

1BDJ の概要

• エントリーDOI: 10.2210/pdb1bdj/pdb
• 分子名称: CHEY, AEROBIC RESPIRATION CONTROL SENSOR PROTEIN ARCB, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: two-component system, histidine-containing phosphotransfer (hpt) domain, arcb, response regulator, chey, complex (chemotaxis-transferase), complex (chemotaxis-transferase) complex, complex (chemotaxis/transferase)
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28091.14

構造登録者

Kato, M.,Mizuno, T.,Shimizu, T.,Hakoshima, T. (登録日: 1998-05-10, 公開日: 1999-05-11, 最終更新日: 2024-05-22)

主引用文献

Kato, M.,Shimizu, T.,Mizuno, T.,Hakoshima, T.
Structure of the histidine-containing phosphotransfer (HPt) domain of the anaerobic sensor protein ArcB complexed with the chemotaxis response regulator CheY.
Acta Crystallogr.,Sect.D, 55:1257-1263, 1999

PubMed Abstract: The three-dimensional structure of the HPt domain of ArcB complexed with CheY has been determined using the molecular-replacement method. The structure was refined to a crystallographic R factor of 18.3% at 2.68 A resolution. The final model included 1899 protein atoms (117 residues from the HPt domain and 128 residues from CheY), one sulfate ion and 44 solvent molecules. In the crystal, CheY molecules stacked along the a axis of the cell with no interactions between neighbouring rows and the HPt domain bridged the CheY molecules. The phosphodonor residue His715 was fully exposed to the solvent region, even though the HPt domain was in contact with four molecules of CheY. CheY showed significant conformational change. This indicates that the HPt domain has a rigid structure when complexed with CheY.

PubMed: 10393292
DOI: 10.1107/S0907444999005053

実験手法

X-RAY DIFFRACTION (2.68 Å)