1BD4

UPRT-URACIL COMPLEX

1BD4 の概要

• エントリーDOI: 10.2210/pdb1bd4/pdb
• 分子名称: URACIL PHOSPHORIBOSYLTRANSFERASE, PHOSPHATE ION, URACIL, ... (4 entities in total)
• 機能のキーワード: transferase, uprt, uracil, glycosyltransferase, complex
• 由来する生物種: Toxoplasma gondii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 111441.10

構造登録者

Schumacher, M.A.,Carter, D.,Scott, D.,Roos, D.,Ullman, B.,Brennan, R.G. (登録日: 1998-05-12, 公開日: 1999-05-18, 最終更新日: 2024-02-07)

主引用文献

Schumacher, M.A.,Carter, D.,Scott, D.M.,Roos, D.S.,Ullman, B.,Brennan, R.G.
Crystal structures of Toxoplasma gondii uracil phosphoribosyltransferase reveal the atomic basis of pyrimidine discrimination and prodrug binding.
EMBO J., 17:3219-3232, 1998

PubMed Abstract: Uracil phosphoribosyltransferase (UPRTase) catalyzes the transfer of a ribosyl phosphate group from alpha-D-5-phosphoribosyl-1-pyrophosphate to the N1 nitrogen of uracil. The UPRTase from the opportunistic pathogen Toxoplasma gondii is a rational target for antiparasitic drug design. To aid in structure-based drug design studies against toxoplasmosis, the crystal structures of the T.gondii apo UPRTase (1.93 A resolution), the UPRTase bound to its substrate, uracil (2.2 A resolution), its product, UMP (2.5 A resolution), and the prodrug, 5-fluorouracil (2.3 A resolution), have been determined. These structures reveal that UPRTase recognizes uracil through polypeptide backbone hydrogen bonds to the uracil exocyclic O2 and endocyclic N3 atoms and a backbone-water-exocyclic O4 oxygen hydrogen bond. This stereochemical arrangement and the architecture of the uracil-binding pocket reveal why cytosine and pyrimidines with exocyclic substituents at ring position 5 larger than fluorine, including thymine, cannot bind to the enzyme. Strikingly, the T. gondii UPRTase contains a 22 residue insertion within the conserved PRTase fold that forms an extended antiparallel beta-arm. Leu92, at the tip of this arm, functions to cap the active site of its dimer mate, thereby inhibiting the escape of the substrate-binding water molecule.

PubMed: 9628859
DOI: 10.1093/emboj/17.12.3219

実験手法

X-RAY DIFFRACTION (2.2 Å)