1BCO

BACTERIOPHAGE MU TRANSPOSASE CORE DOMAIN

1BCO の概要

• エントリーDOI: 10.2210/pdb1bco/pdb
• 分子名称: BACTERIOPHAGE MU TRANSPOSASE (2 entities in total)
• 機能のキーワード: polynucleotidyl transferase, dna binding, endonuclease, integrase, transposase
• 由来する生物種: Enterobacteria phage Mu
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36888.08

構造登録者

Rice, P.A.,Mizuuchi, K. (登録日: 1995-05-26, 公開日: 1995-10-15, 最終更新日: 2024-02-07)

主引用文献

Rice, P.,Mizuuchi, K.
Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration.
Cell(Cambridge,Mass.), 82:209-220, 1995

PubMed Abstract: The crystal structure of the core domain of bacteriophage Mu transposase, MuA, has been determined at 2.4 A resolution. The first of two subdomains contains the active site and, despite very limited sequence homology, exhibits a striking similarity to the core domain of HIV-1 integrase, which carries out a similar set of biochemical reactions. It also exhibits more limited similarity to other nucleases, RNase H and RuvC. The second, a beta barrel, connects to the first subdomain through several contacts. Three independent determinations of the monomer structure from two crystal forms all show the active site held in a similar, apparently inactive configuration. The enzymatic activity of MuA is known to be activated by formation of a DNA-bound tetramer of the protein. We propose that the connections between the two subdomains may be involved in the cross-talk between the active site and the other domains of the transposase that controls the activity of the protein.

PubMed: 7628012
DOI: 10.1016/0092-8674(95)90308-9

実験手法

X-RAY DIFFRACTION (2.4 Å)