1BBT

METHODS USED IN THE STRUCTURE DETERMINATION OF FOOT AND MOUTH DISEASE VIRUS

1BBT の概要

• エントリーDOI: 10.2210/pdb1bbt/pdb
• 分子名称: FOOT-AND-MOUTH DISEASE VIRUS (SUBUNIT VP1), FOOT-AND-MOUTH DISEASE VIRUS (SUBUNIT VP2), FOOT-AND-MOUTH DISEASE VIRUS (SUBUNIT VP3), ... (4 entities in total)
• 機能のキーワード: virus, icosahedral virus
• 由来する生物種: Foot-and-mouth disease virus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 80826.57

構造登録者

Acharya, K.R.,Fry, E.E.,Logan, D.T.,Stuart, D.I. (登録日: 1992-05-18, 公開日: 1994-01-31, 最終更新日: 2024-02-07)

主引用文献

Fry, E.,Acharya, R.,Stuart, D.
Methods used in the structure determination of foot-and-mouth disease virus.
Acta Crystallogr.,Sect.A, 49:45-55, 1993

PubMed Abstract: The structure of foot-and-mouth disease virus (FMDV) strain O1 BFS 1860 has been determined to 2.9 A resolution using the molecular-replacement method [Acharya, Fry, Stuart, Fox, Rowlands & Brown (1989). Nature (London), 337, 709-716]. Crystals of the virus with average dimensions 0.12 x 0.06 x 0.12 mm belong to space group I23, a = 345 A with 1/12 of the icosahedral particle per asymmetric unit giving fivefold noncrystallographic redundancy. Oscillation diffraction photographs were collected at the SERC Synchrotron Radiation Source at Daresbury in accordance with strict disease security regulations. The ambiguity in particle orientation was resolved using a self-rotation function and starting estimates of the phases to 8 A were derived from the known structures of two picornaviruses similarly oriented in the I23 unit cell. The phases were refined and extended using iterative averaging and solvent flattening with the implementation of a simple automatic envelope-determination procedure to increase the phasing power available.

PubMed: 8382928
DOI: 10.1107/S0108767392005737

実験手法

X-RAY DIFFRACTION (2.6 Å)