1BBO

HIGH-RESOLUTION SOLUTION STRUCTURE OF THE DOUBLE CYS2*HIS2 ZINC FINGER FROM THE HUMAN ENHANCER BINDING PROTEIN MBP-1

1BBO の概要

• エントリーDOI: 10.2210/pdb1bbo/pdb
• 分子名称: HUMAN ENHANCER-BINDING PROTEIN MBP-1, ZINC ION (2 entities in total)
• 機能のキーワード: dna-binding protein, dna binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6904.92

構造登録者

Clore, G.M.,Omichinski, J.G.,Gronenborn, A.M. (登録日: 1992-05-01, 公開日: 1993-10-31, 最終更新日: 2023-11-15)

主引用文献

Omichinski, J.G.,Clore, G.M.,Robien, M.,Sakaguchi, K.,Appella, E.,Gronenborn, A.M.
High-resolution solution structure of the double Cys2His2 zinc finger from the human enhancer binding protein MBP-1.
Biochemistry, 31:3907-3917, 1992

PubMed Abstract: The high-resolution three-dimensional structure of a synthetic 57-residue peptide comprising the double zinc finger of the human enhancer binding protein MBP-1 has been determined in solution by nuclear magnetic resonance spectroscopy on the basis of 1280 experimental restraints. A total of 30 simulated annealing structures were calculated. The backbone atomic root-mean-square distributions about the mean coordinate positions are 0.32 and 0.33 A for the N- and C-terminal fingers, respectively, and the corresponding values for all atoms, excluding disordered surface side chains, are 0.36 and 0.40 A. Each finger comprises an irregular antiparallel sheet and a helix, with the zinc tetrahedrally coordinated to two cysteines and two histidines. The overall structure is nonglobular in nature, and the angle between the long axes of the helices is 47 +/- 5 degrees. The long axis of the antiparallel sheet in the N-terminal finger is approximately parallel to that of the helix in the C-terminal finger. Comparison of this structure with the X-ray structure of the Zif-268 triple finger complexed with DNA indicates that the relative orientation of the individual zinc fingers is clearly distinct in the two cases. This difference can be attributed to the presence of a long Lys side chain in the C-terminal finger of MBP-1 at position 40, instead of a short Ala or Ser side chain at the equivalent position in Zif-268. This finding suggests that different contacts may be involved in the binding of the zinc fingers of MBP-1 and Zif-268 to DNA, consistent with the findings from methylation interference experiments that the two fingers of MBP-1 contact 10 base pairs, while the three fingers of Zif-268 contact only 9 base pairs.

PubMed: 1567844
DOI: 10.1021/bi00131a004

実験手法

SOLUTION NMR