1BAU

NMR STRUCTURE OF THE DIMER INITIATION COMPLEX OF HIV-1 GENOMIC RNA, MINIMIZED AVERAGE STRUCTURE

1BAU の概要

• エントリーDOI: 10.2210/pdb1bau/pdb
• 分子名称: SL1 RNA DIMER (1 entity in total)
• 機能のキーワード: ribonucleic acid, hiv-1, dimerization, encapsidation, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14834.98

構造登録者

Mujeeb, A.,Clever, J.L.,Billeci, T.M.,James, T.L.,Parslow, T.G. (登録日: 1998-04-18, 公開日: 1999-04-27, 最終更新日: 2024-05-22)

主引用文献

Mujeeb, A.,Clever, J.L.,Billeci, T.M.,James, T.L.,Parslow, T.G.
Structure of the dimer initiation complex of HIV-1 genomic RNA.
Nat.Struct.Biol., 5:432-436, 1998

PubMed Abstract: Retroviral genomes must dimerize to be fully infectious. Dimerization is directed by a unique RNA hairpin structure with a palindrome in its loop: hairpins of two strands first associate transiently through their loops, and then refold to a more stable, linear duplex. The structure of the initial, kissing-loop dimer from HIV-1, solved using 2D NMR, is bent and metastable, its interface being formed not only by standard basepairing between palindromes, but also by a distinctive pattern of interstrand stacking among bases at the stem-loop junctions. This creates mechanical distortions that partially melt both stems, which may facilitate spontaneous refolding of this RNA complex into linear form.

PubMed: 9628479
DOI: 10.1038/nsb0698-432

実験手法

SOLUTION NMR