1BA5

DNA-BINDING DOMAIN OF HUMAN TELOMERIC PROTEIN, HTRF1, NMR, 18 STRUCTURES

1BA5 の概要

• エントリーDOI: 10.2210/pdb1ba5/pdb
• NMR情報: BMRB: 4210
• 分子名称: HTRF1 (1 entity in total)
• 機能のキーワード: dna-binding domain, myb repeats, telomeres, trf
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P54274
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6668.81

構造登録者

Nishikawa, T.,Nagadoi, A.,Yoshimura, S.,Aimoto, S.,Nishimura, Y. (登録日: 1998-04-22, 公開日: 1999-04-27, 最終更新日: 2024-05-22)

主引用文献

Nishikawa, T.,Nagadoi, A.,Yoshimura, S.,Aimoto, S.,Nishimura, Y.
Solution structure of the DNA-binding domain of human telomeric protein, hTRF1.
Structure, 6:1057-1065, 1998

PubMed Abstract: Mammalian telomeres consist of long tandem arrays of the double-stranded TTAGGG sequence motif packaged by a telomere repeat binding factor, TRF1. The DNA-binding domain of TRF1 shows sequence homology to each of three tandem repeats of the DNA-binding domain of the transcriptional activator c-Myb. The isolated c-Myb-like domain of human TRF1 (hTRF1) binds specifically to telomeric DNA as a monomer, in a similar manner to that of homeodomains. So far, the only three-dimensional structure of a telomeric protein to be determined is that of a yeast telomeric protein, Rap 1p. The DNA-binding domain of Rap 1p contains two subdomains that are structurally closely related to c-Myb repeats. We set out to determine the solution structure of the DNA-binding domain of hTRF1 in order to establish its mode of DNA binding.

PubMed: 9739097
DOI: 10.1016/S0969-2126(98)00106-3

実験手法

SOLUTION NMR