1BA3

FIREFLY LUCIFERASE IN COMPLEX WITH BROMOFORM

1BA3 の概要

• エントリーDOI: 10.2210/pdb1ba3/pdb
• 分子名称: LUCIFERASE, TRIBROMOMETHANE (3 entities in total)
• 機能のキーワード: oxidoreductase, monooxygenase, photoprotein, luminescence
• 由来する生物種: Photinus pyralis (common eastern firefly)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 61324.42

構造登録者

Franks, N.P.,Jenkins, A.,Conti, E.,Lieb, W.R.,Brick, P. (登録日: 1998-04-21, 公開日: 1998-11-11, 最終更新日: 2024-05-22)

主引用文献

Franks, N.P.,Jenkins, A.,Conti, E.,Lieb, W.R.,Brick, P.
Structural basis for the inhibition of firefly luciferase by a general anesthetic.
Biophys.J., 75:2205-2211, 1998

PubMed Abstract: The firefly luciferase enzyme from Photinus pyralis is probably the best-characterized model system for studying anesthetic-protein interactions. It binds a diverse range of general anesthetics over a large potency range, displays a sensitivity to anesthetics that is very similar to that found in animals, and has an anesthetic sensitivity that can be modulated by one of its substrates (ATP). In this paper we describe the properties of bromoform acting as a general anesthetic (in Rana temporaria tadpoles) and as an inhibitor of the firefly luciferase enzyme at high and low ATP concentrations. In addition, we describe the crystal structure of the low-ATP form of the luciferase enzyme in the presence of bromoform at 2.2-A resolution. These results provide a structural basis for understanding the anesthetic inhibition of the enzyme, as well as an explanation for the ATP modulation of its anesthetic sensitivity.

PubMed: 9788915

実験手法

X-RAY DIFFRACTION (2.2 Å)