1B93

METHYLGLYOXAL SYNTHASE FROM ESCHERICHIA COLI

1B93 の概要

• エントリーDOI: 10.2210/pdb1b93/pdb
• 分子名称: PROTEIN (METHYLGLYOXAL SYNTHASE), FORMIC ACID, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: glycolytic bypass, methylglyoxal, lyase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P0A731
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 51229.78

構造登録者

Saadat, D.,Harrison, D.H.T. (登録日: 1999-02-23, 公開日: 1999-03-16, 最終更新日: 2023-12-27)

主引用文献

Saadat, D.,Harrison, D.H.
The crystal structure of methylglyoxal synthase from Escherichia coli.
Structure Fold.Des., 7:309-317, 1999

PubMed Abstract: The reaction mechanism of methylglyoxal synthase (MGS) is believed to be similar to that of triosephosphate isomerase (TIM). Both enzymes utilise dihydroxyacetone phosphate (DHAP) to form an enediol(ate) phosphate intermediate as the first step of their reaction pathways. However, the second catalytic step in the MGS reaction pathway is characterized by the elimination of phosphate and collapse of the enediol(ate) to form methylglyoxal instead of reprotonation to form the isomer glyceraldehyde 3-phosphate.

PubMed: 10368300
DOI: 10.1016/S0969-2126(99)80041-0

実験手法

X-RAY DIFFRACTION (1.9 Å)