1B8Z

HU FROM THERMOTOGA MARITIMA

Summary for 1B8Z

• Entry DOI: 10.2210/pdb1b8z/pdb
• Descriptor: PROTEIN (HISTONELIKE PROTEIN HU) (2 entities in total)
• Functional Keywords: thermotoga maritima, thermostable dna binding protein, dna binding protein
• Biological source: Thermotoga maritima
• Total number of polymer chains: 2
• Total formula weight: 20036.20

Authors

Christodoulou, E.,Rypniewski, W.R.,Vorgias, C.E. (deposition date: 1999-02-03, release date: 2000-02-03, Last modification date: 2023-12-27)

Primary citation

Christodoulou, E.,Vorgias, C.E.
Cloning, overproduction, purification and crystallization of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima.
Acta Crystallogr.,Sect.D, 54:1043-1045, 1998

PubMed Abstract: The humar gene encoding for the histone-like DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima was efficiently overexpressed in Escherichia coli under the T7 promoter. The HU protein was purified using SP-Sepharose ion-exchange and heparin-affinity chromatography and was successfully crystallized in ammonium sulfate. The crystals were grown in the tetragonal form in space group P43 or P41 and have unit-cell dimensions a = b = 46.12, c = 77.56 A, alpha = beta = gamma = 90 degrees. The crystals diffract X-rays to 1.6 A resolution using synchrotron radiation and are suitable for determination of the HU structure at high resolution.

PubMed: 9757133
DOI: 10.1107/S0907444998000341

Experimental method

X-RAY DIFFRACTION (1.6 Å)