1B8Z

HU FROM THERMOTOGA MARITIMA

1B8Z の概要

• エントリーDOI: 10.2210/pdb1b8z/pdb
• 分子名称: PROTEIN (HISTONELIKE PROTEIN HU) (2 entities in total)
• 機能のキーワード: thermotoga maritima, thermostable dna binding protein, dna binding protein
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20036.20

構造登録者

Christodoulou, E.,Rypniewski, W.R.,Vorgias, C.E. (登録日: 1999-02-03, 公開日: 2000-02-03, 最終更新日: 2023-12-27)

主引用文献

Christodoulou, E.,Vorgias, C.E.
Cloning, overproduction, purification and crystallization of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima.
Acta Crystallogr.,Sect.D, 54:1043-1045, 1998

PubMed Abstract: The humar gene encoding for the histone-like DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima was efficiently overexpressed in Escherichia coli under the T7 promoter. The HU protein was purified using SP-Sepharose ion-exchange and heparin-affinity chromatography and was successfully crystallized in ammonium sulfate. The crystals were grown in the tetragonal form in space group P43 or P41 and have unit-cell dimensions a = b = 46.12, c = 77.56 A, alpha = beta = gamma = 90 degrees. The crystals diffract X-rays to 1.6 A resolution using synchrotron radiation and are suitable for determination of the HU structure at high resolution.

PubMed: 9757133
DOI: 10.1107/S0907444998000341

実験手法

X-RAY DIFFRACTION (1.6 Å)