1B8D

CRYSTAL STRUCTURE OF A PHYCOUROBILIN-CONTAINING PHYCOERYTHRIN

1B8D の概要

• エントリーDOI: 10.2210/pdb1b8d/pdb
• 分子名称: PROTEIN (RHODOPHYTAN PHYCOERYTHRIN (ALPHA CHAIN)), PROTEIN (RHODOPHYTAN PHYCOERYTHRIN (BETA CHAIN)), PROTEIN (RHODOPHYTAN PHYCOERYTHRIN (GAMMA CHAIN)), ... (6 entities in total)
• 機能のキーワード: light-harvesting complex, red algae, phycobiliprotein, photosynthesis
• 由来する生物種: Griffithsia monilis; 詳細
• 細胞内の位置: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side: O36005; Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side (By similarity): O36004
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 78947.25

構造登録者

Ritter, S.,Hiller, R.G.,Wrench, P.M.,Welte, W.,Diederichs, K. (登録日: 1999-01-29, 公開日: 1999-02-18, 最終更新日: 2023-08-09)

主引用文献

Ritter, S.,Hiller, R.G.,Wrench, P.M.,Welte, W.,Diederichs, K.
Crystal structure of a phycourobilin-containing phycoerythrin at 1.90-A resolution.
J.Struct.Biol., 126:86-97, 1999

PubMed Abstract: The structure of R-phycoerythrin (R-PE) from the red alga Griffithsia monilis was solved at 1.90-A resolution by molecular replacement, using the atomic coordinates of cyanobacterial phycocyanin from Fremyella diplosiphon as a model. The crystallographic R factor for the final model is 17.5% (Rfree 22.7%) for reflections in the range 100-1.90 A. The model consists of an (alphabeta)2 dimer with an internal noncrystallographic dyad and a fragment of the gamma-polypeptide. The alpha-polypeptide (164 amino acid residues) has two covalently bound phycoerythrobilins at positions alpha82 and alpha139. The beta-polypeptide (177 residues) has two phycoerythrobilins bound to residues beta82 and beta158 and one phycourobilin covalently attached to rings A and D at residues beta50 and beta61, respectively. The electron density of the gamma-polypeptide is mostly averaged out by threefold crystallographic symmetry, but a dipeptide (Gly-Tyr) and one single Tyr could be modeled. These two tyrosine residues of the gamma-polypeptide are in close proximity to the phycoerythrobilins at position beta82 of two symmetry-related beta-polypeptides and are related by the same noncrystallographic dyad as the (alphabeta)2 dimer. Possible energy transfer pathways are discussed briefly.

PubMed: 10388620
DOI: 10.1006/jsbi.1999.4106

実験手法

X-RAY DIFFRACTION (1.9 Å)