1B85

LIGNIN PEROXIDASE

1B85 の概要

• エントリーDOI: 10.2210/pdb1b85/pdb
• 分子名称: Ligninase H8, CALCIUM ION, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• 機能のキーワード: lignin degradation, heme, radical reaction, electron transfer, oxidoreductase, calcium, cleavage on pair of basic residues, disulfide bond, glycoprotein, hydrogen peroxide, iron, metal-binding, peroxidase, zymogen
• 由来する生物種: Phanerochaete chrysosporium (White-rot fungus)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 76276.65

構造登録者

Blodig, W.,Doyle, W.A.,Smith, A.T.,Piontek, K. (登録日: 1999-02-05, 公開日: 1999-02-16, 最終更新日: 2024-10-30)

主引用文献

Blodig, W.,Smith, A.T.,Doyle, W.A.,Piontek, K.
Crystal structures of pristine and oxidatively processed lignin peroxidase expressed in Escherichia coli and of the W171F variant that eliminates the redox active tryptophan 171. Implications for the reaction mechanism.
J.Mol.Biol., 305:851-861, 2001

PubMed Abstract: The heme enzyme lignin peroxidase (LiP) from the white rot fungus Phanerochaete chrysosporium contains a solvent exposed redox active tryptophan residue (Trp171) that carries a unique hydroxy group stereo-specifically attached to its C(beta) atom. A Trp171Phe mutant has no activity at all towards the substrate veratryl alcohol. The mechanism of veratryl alcohol oxidation involving beta-hydroxy-Trp171 is largely unknown. Here, we present the first crystal structures of LiP isozyme H8 at high resolution in its pristine non-hydroxylated form, of the C(beta)-hydroxylated form, and of the Trp171Phe mutant using recombinantly expressed and refolded protein produced from Escherichia coli. As a consequence, all structures are unglycosylated. Structural changes in response to the mutation are marginal and allow us to attribute the complete lack of activity exclusively to the absence of the redox active indole side-chain. The origin of the stereospecificity of the Trp171 hydroxylation can be explained on structural grounds. A reaction mechanism involving Trp171 is proposed and the possible function of the modification is discussed. Another important result regarding the ongoing debate on the co-ordination state of the heme iron in the resting state is that the iron is six co-ordinate in all cases the data being collected at room temperature. The mean distance from the iron to the distal water ligand is 2.18(+/-0.08) A. The radical scavenger orcinol was found to decrease radiation damage to the crystals, during data collection at room temperature.

PubMed: 11162097
DOI: 10.1006/jmbi.2000.4346

実験手法

X-RAY DIFFRACTION (1.85 Å)