1B7T

MYOSIN DIGESTED BY PAPAIN

Summary for 1B7T

• Entry DOI: 10.2210/pdb1b7t/pdb
• Descriptor: MYOSIN HEAVY CHAIN, MYOSIN REGULATORY LIGHT CHAIN, MYOSIN ESSENTIAL LIGHT CHAIN, ... (7 entities in total)
• Functional Keywords: myosin motor, myosin
• Biological source: Argopecten irradians; More
• Cellular location: Cytoplasm, myofibril: P24733
• Total number of polymer chains: 3
• Total formula weight: 131029.81

Authors

Houdusse, A.,Kalabokis, V.,Himmel, D.,Szent-Gyorgyi, A.G.,Cohen, C. (deposition date: 1999-01-15, release date: 1999-05-12, Last modification date: 2023-09-20)

Primary citation

Houdusse, A.,Kalabokis, V.N.,Himmel, D.,Szent-Gyorgyi, A.G.,Cohen, C.
Atomic structure of scallop myosin subfragment S1 complexed with MgADP: a novel conformation of the myosin head.
Cell(Cambridge,Mass.), 97:459-470, 1999

PubMed Abstract: The crystal structure of a proteolytic subfragment from scallop striated muscle myosin, complexed with MgADP, has been solved at 2.5 A resolution and reveals an unusual conformation of the myosin head. The converter and the lever arm are in very different positions from those in either the pre-power stroke or near-rigor state structures; moreover, in contrast to these structures, the SH1 helix is seen to be unwound. Here we compare the overall organization of the myosin head in these three states and show how the conformation of three flexible "joints" produces rearrangements of the four major subdomains in the myosin head with different bound nucleotides. We believe that this novel structure represents one of the prehydrolysis ("ATP") states of the contractile cycle in which the myosin heads stay detached from actin.

PubMed: 10338210
DOI: 10.1016/S0092-8674(00)80756-4

Experimental method

X-RAY DIFFRACTION (2.5 Å)