1B7T

MYOSIN DIGESTED BY PAPAIN

1B7T の概要

• エントリーDOI: 10.2210/pdb1b7t/pdb
• 分子名称: MYOSIN HEAVY CHAIN, MYOSIN REGULATORY LIGHT CHAIN, MYOSIN ESSENTIAL LIGHT CHAIN, ... (7 entities in total)
• 機能のキーワード: myosin motor, myosin
• 由来する生物種: Argopecten irradians; 詳細
• 細胞内の位置: Cytoplasm, myofibril: P24733
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 131029.81

構造登録者

Houdusse, A.,Kalabokis, V.,Himmel, D.,Szent-Gyorgyi, A.G.,Cohen, C. (登録日: 1999-01-15, 公開日: 1999-05-12, 最終更新日: 2023-09-20)

主引用文献

Houdusse, A.,Kalabokis, V.N.,Himmel, D.,Szent-Gyorgyi, A.G.,Cohen, C.
Atomic structure of scallop myosin subfragment S1 complexed with MgADP: a novel conformation of the myosin head.
Cell(Cambridge,Mass.), 97:459-470, 1999

PubMed Abstract: The crystal structure of a proteolytic subfragment from scallop striated muscle myosin, complexed with MgADP, has been solved at 2.5 A resolution and reveals an unusual conformation of the myosin head. The converter and the lever arm are in very different positions from those in either the pre-power stroke or near-rigor state structures; moreover, in contrast to these structures, the SH1 helix is seen to be unwound. Here we compare the overall organization of the myosin head in these three states and show how the conformation of three flexible "joints" produces rearrangements of the four major subdomains in the myosin head with different bound nucleotides. We believe that this novel structure represents one of the prehydrolysis ("ATP") states of the contractile cycle in which the myosin heads stay detached from actin.

PubMed: 10338210
DOI: 10.1016/S0092-8674(00)80756-4

実験手法

X-RAY DIFFRACTION (2.5 Å)