1B4Y
STRUCTURE AND MECHANISM OF FORMATION OF THE H-Y5 ISOMER OF AN INTRAMOLECULAR DNA TRIPLE HELIX.
Summary for 1B4Y
| Entry DOI | 10.2210/pdb1b4y/pdb |
| NMR Information | BMRB: 4400 |
| Descriptor | DNA (H-Y5 TRIPLE HELIX) (1 entity in total) |
| Functional Keywords | h-dna, triple helix, dna |
| Total number of polymer chains | 1 |
| Total formula weight | 9072.82 |
| Authors | Van Dongen, M.J.P.,Doreleijers, J.F.,Van Der Marel, G.A.,Van Boom, J.H.,Hilbers, C.W.,Wijmenga, S.S. (deposition date: 1998-12-30, release date: 1999-09-13, Last modification date: 2023-12-27) |
| Primary citation | van Dongen, M.J.,Doreleijers, J.F.,van der Marel, G.A.,van Boom, J.H.,Hilbers, C.W.,Wijmenga, S.S. Structure and mechanism of formation of the H-y5 isomer of an intramolecular DNA triple helix. Nat.Struct.Biol., 6:854-859, 1999 Cited by PubMed Abstract: H-DNA, thought to play a regulatory role in transcription, exists in two isomeric forms, H-y3 and H-y5. We present the first solution structure of a DNA fragment representing the H-y5 fold. The structure shows the H-y5 triple helix, and for the first time how in an H-DNA isomer the purine strand extension interacts with the triplex loop. It gives direct insight into the mechanism of H-DNA formation, and explains a host of biochemical and biophysical data on the relative stability of the H-DNA isomers. In addition, the observed interaction of the purine strand extension and the triplex loop provides new clues to the design of clamp-type triple helix-forming oligonucleotides. PubMed: 10467098DOI: 10.1038/12313 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
Download full validation report
