1B4Y

STRUCTURE AND MECHANISM OF FORMATION OF THE H-Y5 ISOMER OF AN INTRAMOLECULAR DNA TRIPLE HELIX.

1B4Y の概要

• エントリーDOI: 10.2210/pdb1b4y/pdb
• NMR情報: BMRB: 4400
• 分子名称: DNA (H-Y5 TRIPLE HELIX) (1 entity in total)
• 機能のキーワード: h-dna, triple helix, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9072.82

構造登録者

Van Dongen, M.J.P.,Doreleijers, J.F.,Van Der Marel, G.A.,Van Boom, J.H.,Hilbers, C.W.,Wijmenga, S.S. (登録日: 1998-12-30, 公開日: 1999-09-13, 最終更新日: 2023-12-27)

主引用文献

van Dongen, M.J.,Doreleijers, J.F.,van der Marel, G.A.,van Boom, J.H.,Hilbers, C.W.,Wijmenga, S.S.
Structure and mechanism of formation of the H-y5 isomer of an intramolecular DNA triple helix.
Nat.Struct.Biol., 6:854-859, 1999

PubMed Abstract: H-DNA, thought to play a regulatory role in transcription, exists in two isomeric forms, H-y3 and H-y5. We present the first solution structure of a DNA fragment representing the H-y5 fold. The structure shows the H-y5 triple helix, and for the first time how in an H-DNA isomer the purine strand extension interacts with the triplex loop. It gives direct insight into the mechanism of H-DNA formation, and explains a host of biochemical and biophysical data on the relative stability of the H-DNA isomers. In addition, the observed interaction of the purine strand extension and the triplex loop provides new clues to the design of clamp-type triple helix-forming oligonucleotides.

PubMed: 10467098
DOI: 10.1038/12313

実験手法

SOLUTION NMR