1B4U

PROTOCATECHUATE 4,5-DIOXYGENASE (LIGAB) IN COMPLEX WITH PROTOCATECHUATE (PCA)

1B4U の概要

• エントリーDOI: 10.2210/pdb1b4u/pdb
• 分子名称: PROTOCATECHUATE 4,5-DIOXYGENASE, FE (III) ION, 3,4-DIHYDROXYBENZOIC ACID, ... (5 entities in total)
• 機能のキーワード: extradiol type dioxygenase, protocatechuate, non-heme iron protein, dioxygenase
• 由来する生物種: Sphingomonas paucimobilis; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 98200.95

構造登録者

Sugimoto, K.,Senda, T.,Mitsui, Y. (登録日: 1998-12-29, 公開日: 1999-08-27, 最終更新日: 2024-02-07)

主引用文献

Sugimoto, K.,Senda, T.,Aoshima, H.,Masai, E.,Fukuda, M.,Mitsui, Y.
Crystal structure of an aromatic ring opening dioxygenase LigAB, a protocatechuate 4,5-dioxygenase, under aerobic conditions.
Structure Fold.Des., 7:953-965, 1999

PubMed Abstract: Sphingomonas paucimobilis SYK-6 utilizes an extradiol-type catecholic dioxygenase, the LigAB enzyme (a protocatechuate 4,5-dioxygenase), to oxidize protocatechuate (or 3,4-dihydroxybenzoic acid, PCA). The enzyme belongs to the family of class III extradiol-type catecholic dioxygenases catalyzing the ring-opening reaction of protocatechuate and related compounds. The primary structure of LigAB suggests that the enzyme has no evolutionary relationship with the family of class II extradiol-type catecholic dioxygenases. Both the class II and class III enzymes utilize a non-heme ferrous center for adding dioxygen to the substrate. By elucidating the structure of LigAB, we aimed to provide a structural basis for discussing the function of class III enzymes.

PubMed: 10467151
DOI: 10.1016/S0969-2126(99)80122-1

実験手法

X-RAY DIFFRACTION (2.2 Å)