1B36

SOLUTION STRUCTURE OF THE HAIRPIN RIBOZYME LOOP B DOMAIN RNA, NMR, 10 STRUCTURES

Summary for 1B36

• Entry DOI: 10.2210/pdb1b36/pdb
• Descriptor: RNA (RNA LOOP B) (1 entity in total)
• Functional Keywords: ribonucleic acid, tetraloop receptor, group i intron, group ii intron, rna
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 12215.32

Authors

Butcher, S.E.,Dieckmann, T.,Feigon, J. (deposition date: 1998-12-17, release date: 1998-12-23, Last modification date: 2023-12-27)

Primary citation

Butcher, S.E.,Allain, F.H.,Feigon, J.
Solution structure of the loop B domain from the hairpin ribozyme.
Nat.Struct.Biol., 6:212-216, 1999

PubMed Abstract: The hairpin ribozyme is a small catalytic RNA with a unique two-domain structure. Here we present the solution structure of the loop B domain of the hairpin ribozyme, which contains most of the catalytically essential nucleotides. The 38-nucleotide domain contains a 16-nucleotide internal loop that forms one of the largest non-Watson-Crick segments of base pairing thus far determined by either NMR or crystallography. Since the solution structure of the smaller loop A domain has been previously solved, an NMR structure-based model of the 22,000 Mr hairpin ribozyme-substrate open complex emerges by joining the two domain structures. Strikingly, catalytically essential functional groups for the loop B domain are concentrated within an expanded minor groove, presenting a clear docking surface for the loop A domain.

PubMed: 10074938
DOI: 10.1038/6651

Experimental method

SOLUTION NMR