1B1Z

STREPTOCOCCAL PYROGENIC EXOTOXIN A1

1B1Z の概要

• エントリーDOI: 10.2210/pdb1b1z/pdb
• 分子名称: PROTEIN (TOXIN) (2 entities in total)
• 機能のキーワード: superantigen, zinc binding, pyrogenic exotoxin, toxin
• 由来する生物種: Streptococcus pyogenes
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 102254.94

構造登録者

Papageorgiou, A.C.,Acharya, K.R. (登録日: 1998-11-24, 公開日: 1999-11-24, 最終更新日: 2024-11-20)

主引用文献

Papageorgiou, A.C.,Collins, C.M.,Gutman, D.M.,Kline, J.B.,O'Brien, S.M.,Tranter, H.S.,Acharya, K.R.
Structural basis for the recognition of superantigen streptococcal pyrogenic exotoxin A (SpeA1) by MHC class II molecules and T-cell receptors.
EMBO J., 18:9-21, 1999

PubMed Abstract: Streptococcal pyrogenic exotoxin A (SpeA) is a superantigen produced by Streptococcus pyogenes and is associated with severe infections characterized by rash, hypotension, multiorgan failure and a high mortality rate. In this study, an allelic form of this toxin, SpeA1, was crystallized with four molecules in the crystallographic asymmetric unit and its crystal structure was determined at 2.6 A resolution. The crystallographic R-factor was 19.4% (33 497 reflections) for 7031 protein atoms and 88 water molecules. The overall structure of SpeA1 is considerably similar to that of other prototype microbial superantigens, either of staphylococcal or streptococcal origin, but has greatest similarity to staphylococcal enterotoxin C (SEC). Based on structural and mutagenesis data, we have mapped several important residues on the toxin molecule, which are involved in the recognition of major histocompatibility complex (MHC) class II molecules and T-cell receptors. Also, the toxin appears to possess a potential zinc-binding site which may have implications in binding to particular MHC class II molecules. Finally, we propose models for SpeA1-MHC class II and SpeA1-T-cell receptor association and the relevance of this phenomenon to the superantigenic action of this toxin is considered.

PubMed: 9878045
DOI: 10.1093/emboj/18.1.9

実験手法

X-RAY DIFFRACTION (2.57 Å)