1B0D

Structural effects of monovalent anions on polymorphic lysozyme crystals

1B0D の概要

• エントリーDOI: 10.2210/pdb1b0d/pdb
• 分子名称: LYSOZYME, PARA-TOLUENE SULFONATE (3 entities in total)
• 機能のキーワード: hydrolase, o-glycosyl
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14503.36

構造登録者

Vaney, M.C.,Broutin, I.,Retailleau, P.,Lafont, S.,Hamiaux, C.,Prange, T.,Ries-Kautt, M.,Ducruix, A. (登録日: 1998-11-07, 公開日: 1998-11-11, 最終更新日: 2024-10-30)

主引用文献

Vaney, M.C.,Broutin, I.,Retailleau, P.,Douangamath, A.,Lafont, S.,Hamiaux, C.,Prange, T.,Ducruix, A.,Ries-Kautt, M.
Structural effects of monovalent anions on polymorphic lysozyme crystals.
Acta Crystallogr.,Sect.D, 57:929-940, 2001

PubMed Abstract: Understanding direct salt effects on protein crystal polymorphism is addressed by comparing different crystal forms (triclinic, monoclinic, tetragonal and orthorhombic) for hen, turkey, bob white quail and human lysozymes. Four new structures of hen egg-white lysozyme are reported: crystals grown in the presence of NapTS diffracted to 1.85 A, of NaI to 1.6 A, of NaNO(3) to 1.45 A and of KSCN to 1.63 A. These new structures are compared with previously published structures in order to draw a mapping of the surface of different lysozymes interacting with monovalent anions, such as nitrate, chloride, iodide, bromide and thiocyanate. An analysis of the structural sites of these anions in the various lysozyme structures is presented. This study shows common anion sites whatever the crystal form and the chemical nature of anions, while others seem specific to a given geometry and a particular charge environment induced by the crystal packing.

PubMed: 11418760
DOI: 10.1107/S0907444901004504

実験手法

X-RAY DIFFRACTION (1.84 Å)