1B04

STRUCTURE OF THE ADENYLATION DOMAIN OF AN NAD+ DEPENDENT LIGASE

1B04 の概要

• エントリーDOI: 10.2210/pdb1b04/pdb
• 分子名称: PROTEIN (DNA LIGASE) (2 entities in total)
• 機能のキーワード: ligase, dna replication
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71664.70

構造登録者

Singleton, M.R.,Hakansson, K.,Timson, D.J.,Wigley, D.B. (登録日: 1998-11-16, 公開日: 1999-11-22, 最終更新日: 2024-02-07)

主引用文献

Singleton, M.R.,Hakansson, K.,Timson, D.J.,Wigley, D.B.
Structure of the adenylation domain of an NAD+-dependent DNA ligase.
Structure Fold.Des., 7:35-42, 1999

PubMed Abstract: DNA ligases catalyse phosphodiester bond formation between adjacent bases in nicked DNA, thereby sealing the nick. A key step in the catalytic mechanism is the formation of an adenylated DNA intermediate. The adenyl group is derived from either ATP (in eucaryotes and archaea) or NAD+4 (in bacteria). This difference in cofactor specificity suggests that DNA ligase may be a useful antibiotic target.

PubMed: 10368271
DOI: 10.1016/S0969-2126(99)80007-0

実験手法

X-RAY DIFFRACTION (2.8 Å)