1B03

SOLUTION STRUCTURE OF THE ANTIBODY-BOUND HIV-1IIIB V3 PEPTIDE

1B03 の概要

• エントリーDOI: 10.2210/pdb1b03/pdb
• 分子名称: PROTEIN (P1053 PEPTIDE) (1 entity in total)
• 機能のキーワード: p1053 structure, viral protein
• 細胞内の位置: Virion membrane : Q79428
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2017.41

構造登録者

Tugarinov, V.,Zvi, A.,Levy, R.,Anglister, J. (登録日: 1998-11-17, 公開日: 1998-11-25, 最終更新日: 2023-12-27)

主引用文献

Tugarinov, V.,Zvi, A.,Levy, R.,Anglister, J.
A cis proline turn linking two beta-hairpin strands in the solution structure of an antibody-bound HIV-1IIIB V3 peptide.
Nat.Struct.Biol., 6:331-335, 1999

PubMed Abstract: The refined solution structure of an 18-residue HIV-1IIIB V3 peptide in complex with the Fv fragment of an anti-gp120 antibody reveals an unexpected type VI beta-turn comprising residues RGPG at the center of a beta-hairpin. The central glycine and proline of this turn are linked by a cis peptide bond. The residues of the turn interact extensively with the antibody Fv. 15N[1H] NOE measurements show that the backbone of the peptide, including the central QRGPGR loop, is well ordered in the complex. The solution structure is significantly different from the X-ray structures of HIV-1MN V3 peptides bound to anti-peptide antibodies. These differences could be due to a two-residue (QR) insertion preceding the GPGR sequence in the HIV-1IIIB strain, and the much longer peptide epitope immobilized by the anti-gp120 antibody.

PubMed: 10201400
DOI: 10.1038/7567

実験手法

SOLUTION NMR