1AZQ

HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SAC7D BOUND WITH KINKED DNA DUPLEX

1AZQ の概要

• エントリーDOI: 10.2210/pdb1azq/pdb
• NMR情報: BMRB: 5905,5908
• 分子名称: DNA (5'-D(*GP*TP*AP*AP*TP*TP*AP*C)-3'), PROTEIN (HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SAC7D) (3 entities in total)
• 機能のキーワード: complex (chromatin protein-dna), dna-binding, archea, kinked-dna, minor-groove binding, intercalation, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Sulfolobus acidocaldarius
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 12478.17

構造登録者

Robinson, H.,Gao, Y.-G.,Mccrary, B.S.,Edmondson, S.P.,Shriver, J.W.,Wang, A.H.-J. (登録日: 1997-11-20, 公開日: 1999-01-13, 最終更新日: 2023-08-02)

主引用文献

Robinson, H.,Gao, Y.G.,McCrary, B.S.,Edmondson, S.P.,Shriver, J.W.,Wang, A.H.
The hyperthermophile chromosomal protein Sac7d sharply kinks DNA.
Nature, 392:202-205, 1998

PubMed Abstract: The proteins Sac7d and Sso7d belong to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius and S. solfactaricus, respectively. These proteins are extremely stable to heat, acid and chemical agents. Sac7d binds to DNA without any particular sequence preference and thereby increases its melting temperature by approximately 40 degrees C. We have now solved and refined the crystal structure of Sac7d in complex with two DNA sequences to high resolution. The structures are examples of a nonspecific DNA-binding protein bound to DNA, and reveal that Sac7d binds in the minor groove, causing a sharp kinking of the DNA helix that is more marked than that induced by any sequence-specific DNA-binding proteins. The kink results from the intercalation of specific hydrophobic side chains of Sac7d into the DNA structure, but without causing any significant distortion of the protein structure relative to the uncomplexed protein in solution.

PubMed: 9515968
DOI: 10.1038/32455

実験手法

X-RAY DIFFRACTION (1.94 Å)