1AW2

TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS

Summary for 1AW2

• Entry DOI: 10.2210/pdb1aw2/pdb
• Descriptor: TRIOSEPHOSPHATE ISOMERASE, SULFATE ION (3 entities in total)
• Functional Keywords: isomerase, psychrophilic, vibrio marinus
• Biological source: Moritella marina
• Cellular location: Cytoplasm (Probable): P50921
• Total number of polymer chains: 8
• Total formula weight: 214513.84

Authors

Maes, D.,Zeelen, J.P.,Wierenga, R.K. (deposition date: 1997-10-09, release date: 1998-01-28, Last modification date: 2024-05-22)

Primary citation

Alvarez, M.,Zeelen, J.P.,Mainfroid, V.,Rentier-Delrue, F.,Martial, J.A.,Wyns, L.,Wierenga, R.K.,Maes, D.
Triose-phosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus. Kinetic and structural properties.
J.Biol.Chem., 273:2199-2206, 1998

PubMed Abstract: The purification and characterization of triose-phosphate isomerase from the psychrophilic bacterium Vibrio marinus (vTIM) is described. Crystal structures of the vTIM-sulfate complex and the vTIM-2-phosphoglycolate complex (at a 2.7-A resolution) are also presented. The optimal growth temperature of Vibrio marinus is 15 degrees C. Stability studies show that vTIM is an unstable protein with a half-life of only 10 min at 25 degrees C. The vTIM sequence is most closely related to the sequence of Escherichia coli TIM (eTIM) (66% identity), and several unique structural features described for eTIM are also seen in vTIM, but eTIM is considerably more stable. The Td values of vTIM and eTIM, determined by calorimetric studies, are 41 and 54 degrees C, respectively. Amino acid sequence comparison reveals that vTIM has an alanine in loop 8 (at position 238), whereas all other TIM sequences known to date have a serine. The vTIM mutant A238S was produced and characterized. Compared with wild type, the catalytic efficiency of the A238S mutant is somewhat reduced, and its stability is considerably increased.

PubMed: 9442062
DOI: 10.1074/jbc.273.4.2199

Experimental method

X-RAY DIFFRACTION (2.65 Å)