1AV6

VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M7G CAPPED RNA HEXAMER AND S-ADENOSYLHOMOCYSTEINE

Summary for 1AV6

• Entry DOI: 10.2210/pdb1av6/pdb
• Descriptor: RNA (5'-R(*GP*AP*AP*AP*AP*A)-3'), Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase, 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: single-stranded rna, methyltransferase, rna cap, poly(a) polymerase, vaccinia, mrna processing, transcription, complex (transferase-rna), transferase-rna complex, transferase/rna
• Biological source: Vaccinia virus WR (VACV)
• Total number of polymer chains: 2
• Total formula weight: 37442.92

Authors

Hodel, A.E.,Gershon, P.D.,Quiocho, F.A. (deposition date: 1997-09-26, release date: 1998-02-25, Last modification date: 2023-08-02)

Primary citation

Hodel, A.E.,Gershon, P.D.,Quiocho, F.A.
Structural basis for sequence-nonspecific recognition of 5'-capped mRNA by a cap-modifying enzyme.
Mol.Cell, 1:443-447, 1998

PubMed Abstract: Sequence-nonspecific binding of RNA, recognition of a 7-methylguanosine 5' mRNA cap, and methylation of a nucleic acid backbone are three crucial and ubiquitous events in eukaryotic nucleic acid processing and function. These three events occur concurrently in the modification of vaccinia transcripts by the methyltransferase VP39. We report the crystal structure of a ternary complex comprising VP39, coenzyme product S-adenosylhomocysteine, and a 5' m7 G-capped, single-stranded RNA hexamer. This structure reveals a novel and general mechanism for sequence-non-specific recognition of the mRNA transcript in which the protein interacts solely with the sugar-phosphate backbone of a short, single-stranded RNA helix. This report represents the first direct and detailed view of a protein complexed with single-stranded RNA or 5'-capped mRNA.

PubMed: 9660928
DOI: 10.1016/S1097-2765(00)80044-1

Experimental method

X-RAY DIFFRACTION (2.8 Å)