1AV6

VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M7G CAPPED RNA HEXAMER AND S-ADENOSYLHOMOCYSTEINE

1AV6 の概要

• エントリーDOI: 10.2210/pdb1av6/pdb
• 分子名称: RNA (5'-R(*GP*AP*AP*AP*AP*A)-3'), Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase, 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: single-stranded rna, methyltransferase, rna cap, poly(a) polymerase, vaccinia, mrna processing, transcription, complex (transferase-rna), transferase-rna complex, transferase/rna
• 由来する生物種: Vaccinia virus WR (VACV)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37442.92

構造登録者

Hodel, A.E.,Gershon, P.D.,Quiocho, F.A. (登録日: 1997-09-26, 公開日: 1998-02-25, 最終更新日: 2023-08-02)

主引用文献

Hodel, A.E.,Gershon, P.D.,Quiocho, F.A.
Structural basis for sequence-nonspecific recognition of 5'-capped mRNA by a cap-modifying enzyme.
Mol.Cell, 1:443-447, 1998

PubMed Abstract: Sequence-nonspecific binding of RNA, recognition of a 7-methylguanosine 5' mRNA cap, and methylation of a nucleic acid backbone are three crucial and ubiquitous events in eukaryotic nucleic acid processing and function. These three events occur concurrently in the modification of vaccinia transcripts by the methyltransferase VP39. We report the crystal structure of a ternary complex comprising VP39, coenzyme product S-adenosylhomocysteine, and a 5' m7 G-capped, single-stranded RNA hexamer. This structure reveals a novel and general mechanism for sequence-non-specific recognition of the mRNA transcript in which the protein interacts solely with the sugar-phosphate backbone of a short, single-stranded RNA helix. This report represents the first direct and detailed view of a protein complexed with single-stranded RNA or 5'-capped mRNA.

PubMed: 9660928
DOI: 10.1016/S1097-2765(00)80044-1

実験手法

X-RAY DIFFRACTION (2.8 Å)